MATP在不同毛色羊驼皮肤中的表达、定位及与其他基因的相关性分析

doi:10.11843/j.issn.0366-6964.2014.03.023

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 502-508.doi: 10.11843/j.issn.0366-6964.2014.03.023

• 研究简报 • 上一篇

MATP在不同毛色羊驼皮肤中的表达、定位及与其他基因的相关性分析

冀元凯¹，田雪^1,2，范瑞文¹，于秀菊¹，赵园园¹，马淑慧¹，董常生^1*

(1.山西农业大学动物科技学院，太谷 030801； 2.河南师范大学水产学院，新乡 453007)

收稿日期:2013-08-14 出版日期:2014-03-23 发布日期:2014-03-23
通讯作者: 董常生,教授，Tel:0354-6288208，E-mail：cs_dong@sxau.edu.cn
作者简介:冀元凯(1989-)，男，山西太原人，硕士生，主要从事羊驼毛色基因的研究,E-mail：codecass@163.com
基金资助:
国家“863”计划（2013AA102506）；农业部“948”项目（2011-Z33）；山西农业大学科技创新基金（201204）

Study on the Expression and Localization of MATP in Different Colors of Alpaca Skin and the Correlation Analysis of Other Genes

JI Yuan-kai¹,TIAN Xue^1，2，FAN Rui-wen¹，YU Xiu-ju¹,ZHAO Yuan-yuan¹，MA Shu-hui¹，DONG Chang-sheng^1*

(1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801, China; 2.College of Fisheries,Henan Normal University，Xinxiang 453007, China)

Received:2013-08-14 Online:2014-03-23 Published:2014-03-23

摘要/Abstract

摘要：

本研究旨在探索膜相关转运蛋白（MATP）与羊驼毛色形成的相关性。以白色和棕色的成年羊驼为研究对象，主要用荧光定量PCR技术分析MATP基因在不同毛色羊驼皮肤的相对表达量、免疫组织化学法和Western blot对MATP蛋白在不同毛色羊驼皮肤中的定位和表达进行研究。荧光定量PCR结果显示，棕色羊驼中MATP的mRNA相对表达量是白色羊驼的3.047倍（P<0.05）；Western blot结果表明，羊驼皮肤组织总蛋白提取物中存在相对分子质量约53 ku的产物，棕色羊驼的皮肤平均蛋白表达量显著高于白色羊驼（P<0.05）；免疫组化结果显示，MATP在羊驼皮肤毛囊的表皮、根鞘及毛球部均表达。MATP与黑色素细胞分布及含量关系密切，可能通过调控黑色素生成从而参与羊驼毛色形成过程。

Abstract:

The objective of this study was to explore the relationship between MATP and alpaca coat color formation.Real-time quantitative PCR，western blotting and immunohistochemistry were applied for examining the mRNA and protein expression level of MATP in alpaca skin of different coat colors.The gene expression quantity of MATP in brown alpaca’s skin tissue was 3.047 times compared with white alpaca.The protein expression of MATP in brown alpaca skins was significantly higher than that in white alpaca skins（P<0.05）.The MATP was demonstrated to express in epidermis，outer root sheath and dermal papilla of hair follicular by immunohistochemistry.Our findings suggested that MATP was involved in the process of alpaca coat color formation.

中图分类号:

Q786

冀元凯，田雪，范瑞文，于秀菊，赵园园，马淑慧，董常生. MATP在不同毛色羊驼皮肤中的表达、定位及与其他基因的相关性分析[J]. 畜牧兽医学报, 2014, 45(3): 502-508.

JI Yuan-kai,TIAN Xue，FAN Rui-wen，YU Xiu-ju,ZHAO Yuan-yuan，MA Shu-hui，DONG Chang-sheng. Study on the Expression and Localization of MATP in Different Colors of Alpaca Skin and the Correlation Analysis of Other Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 502-508.

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MATP在不同毛色羊驼皮肤中的表达、定位及与其他基因的相关性分析

Study on the Expression and Localization of MATP in Different Colors of Alpaca Skin and the Correlation Analysis of Other Genes

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